Computer simulation giving the consequences of cell-to-cell P-gp activity transfers in co-cultures exposed to doxorubicin, according to the mathematical model. A chemotherapy regimen is simulated by cyclic exposure of an inoculum of 104 cells to 1 μM doxorubicin. The treatment cycled every 14 days, with 10 days of doxorubicin treatment followed by 4 days of interruption, for 4 cycles. The initial proportions of resistant MCF-7/Doxo vary form 0,1% (A & B) to 1% (C & B). Simulations were performed in situations where transfers were abolished (A & C) or permitted (B & D) with the parameters determined in Figure 6. For each condition, the numbers of sensitive, resistant and total cells are plotted versus time. Sensitive MCF-7 are defined as cells having negative growth rate in the presence of 1 μM doxorubicin (see Figure 7).
Pasquier et al. Biology Direct 2011 6:5 doi:10.1186/1745-6150-6-5